Trimethoprim (TMP) shall be dissolved for intraperitoneal injection in mice.
The literature suggests that TMP is soluble in water up to 0.4mg/ml. I tried dissolving 200 mg TMP in 500 ml water, Ringer's lactated solution, or 0.9% normal saline at room temperature. In any of the solutions tested, TMP precipitated and did not fully dissolve.
Any advice is deeply appreciated. If at all possible, I would like to avoid injecting solvents like DMSO - which I've used to dissolve TMP (50mg/ml without issues) for tissue culture applications.
First question is: Which concentration do you need to inject?
If you need the high concentration and it doesn´t even solve in Water, try different pH.
If you can deal with a certain amount of DMSO you can use it as a solubilizer for a stock of much higher concentration and dilute it with water, so DMSO will be maybe few %
Maybe you can use another liquid, what about ethanol?
I don´t know what trimethoprim costs, but try more little amounts, maybe 20mg in 50mL of water.
Good luck
you can use a co-solvent such as PG or PEG, or use a surfactant/blend of surfactants for example polysorbates or SLS
Dear Mr.Kaiser
Kindly check in link
https://www.drugs.com/pro/sulfamethoxazole-and-trimethoprim-injection.html
I hope you get nswer
You may not get good solubility in saline solution because of precipitation or either salting out efffects. You can use various cosolvents like glycerin, PEG-400, less qnty of glycol, or Non ionic surfactants like Tween 80, Tween 20, Span 80.
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