How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Does your study include the real state conditions of developing and under developed countries and provide solutions?

The majority of developing and underdeveloped countries, their citizens, authorities, and governments are in confused states of affairs. Hence failed due to a lack of standards and regulations to...

26 December 2020 1,612 4 View

Do the standard regulations on artificial intelligence put restrictions on the scientific research community ?

With widespread, multinational, accelerated adoption, the pace of adoption of AI and cognitive technology continues unabated. AI adoption by companies and organizations continues to increase, as...

22 November 2020 6,517 6 View

Can a researcher use his previous publications to cite new research that he is working on it?

Rules and regulations regarding this issue are highly welcomed if available.

17 November 2020 196 3 View

What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

Question on Protoplast Media?

Hello All, I really need some help with my protoplast media. I am trying to repeat this cycloheximide chase done in Kang 2003. They reference Tao 2005, where "transfected protoplasts were cultured...

01 February 2020 9,248 1 View

Are there any safety regulations handling Ni/Al reactive multilayers (a.k.a. Ni/Al reactive nanofoils)?

Hello. I'm an undergraduate senior student studying materials science. While doing my winter internship in my department's lab, Ni/Al reactive multilayer has captured my mind. I think its...

01 February 2020 6,142 1 View

For Rosa damascene micropropagation, Use of Which Growth Regulators is to Increase the Number of Shoots Extracted from the Stem Node?

For Rosa damascene micropropagation, Use of Which Growth Regulators is to Increase the Number of Shoots Extracted from the Stem Node?

11 December 2019 7,239 3 View

Research on AI and Cancer Imaging?

I am working on a presentation about AI and cancer imaging ( challenges/ best performing algorthims/ regulations). Can you share any journals or articles that might be helpful? Thank you!

10 November 2019 1,240 6 View

Which regulator of STAT3 can decrease phosphorylation rapidly?

Hi, I have studied in small molecule which decrease phosphorylation of STAT3 in cancer cells. I found that this small molecule decrease entirely the phosphorylation of STAT3 in 5 minutes. But...

02 November 2019 6,278 1 View

Can DC motor be directly connected to solar panel, without using any battery??

I wish to connect a 500w 24V DC motor to solar panels, without using any battery. The rated current of motor is 27.6A. I have two solar panels with 24V and 325 watt ratings.The 'maximum power...

05 October 2019 5,086 6 View