I have read various protocols, but all protocols target only bacterial esterases while I am trying to perform an esterase assay for extracellular fungal esterase. Some protocols use methanol while some use acetonitrile for the preparation of a stock solution of p-NPA.

Moreover, I want to perform the test for crude extract while the papers have performed the test with purified enzymes.

What can be the best approach for performing a p-NPA assay for extracellular fungal esterases?

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