During my enzymatic hydrolysis studies, I used enzyme by taking small amount (e.g 150 microliter of Celluclast 1.5 L to hydrolyze 1 gram corn cob). Is it posssible to convert this microliter to FPU/g glucan or FPU/g dry matter?
At first, ask the manufacturer of the cellulase-mix. Ther must be some data for each single batch of celluclast-preparation..
Secondly, you have to measure it. Urg, i hate this chemical way to measure reducing sugars like the procedure of Mary Mandels. Perhaps change from the merry old FPU's to some other charakteristics to standardise your doses.
Thirdly, as your last chance you change your cellulase manufacturer. Here in Germany are a lot of People selling preparations for enhancement of anaerobic digestion of corn. This will be the same at your side.
Cellulase activities and its conversion is best answered by Ghose method for cellulase analysis with detailed information based on the calculation procedure.
Maybe you will not find FPUs as an information for comercially available enzymes. The test is nice because it is a cheap and simple way to monitor cellulase activities on complete fivers. The standardisation is ok for the research purposes, usually industrial customers will rely more on test with powderd cellulose or pigment bonded carbohydrates like Azo-Celluolse or Azo Xyloglucan from Megazyme, they offer an easier handling and a better reliability, you can also scale them up f.E. in 96-well plates for high throughput-Applications.
If you have to do this very often, I would rely on the measurement that the enzyme supplier uses. If you buy enzymes in large scales, you dont buy per units, you buy per kg or litre, which usally has a guaranteed minimum activity, usually related to a common Cellulase Assay, but mostly not FPU.
It is usually enough if you prove the correlation to you original enzyme activity units to FPU once.