I have recently started working with this strain, and have been running into the issue of low viability on the testing plates. Are there any tips I can follow while working with S.pneumoniae (ATCC6305) ?
In discourse analysis, how are the results shown in percentages in the transitivity of a text in a novel,could you please name a few text analysing free software?
25 December 2023 1,999 5 View
Discourse Analysis- Transitivity framework in novels
12 November 2023 1,070 0 View
Hello RG Community, I need advice about testing moderation and mediation hypotheses with variables measured at different levels. We have data from 18 countries, and I was curious if I can test...
30 August 2020 8,349 3 View
How can I find the value of dipolar relaxation constant (Cdd) for bulk water?
03 May 2020 1,498 0 View
I have to fit long cylinders structures using the SASfit package. I would like to know which are the best reasonings (steps) to make a good fit? What do I have to consider when I'm fitting the...
29 May 2019 8,651 2 View
I usually transfect MCF-7 cells using HiPerfect (qiagen) for miRNA mimics/antimiRs. This time, i would like to try another transfection reagent cheaper than HiPerfect. Do you have experience...
29 October 2018 1,807 2 View
During my enzymatic hydrolysis studies, I used enzyme by taking small amount (e.g 150 microliter of Celluclast 1.5 L to hydrolyze 1 gram corn cob). Is it posssible to convert this microliter to...
19 January 2017 4,510 4 View
We have made total DNA extraction and seen absorbance value in nanodrop, but, we couldn't have a image from gel electrophoresis, and consequently, we thought that DNA was degenerated. Could you...
12 February 2015 529 8 View
Recently I connected my computer to the internet via ethernet cable and now I can no longer connect the computer to the HPLC. The connection between the computer and HPLC system controller is...
23 March 2014 2,058 3 View
Hello. Can anyone clarify if the transitivity of a novel (text) of 2000 words should be conducted word by word when you want to analyse the most prominent process type or Just write the results...
01 January 1970 2,212 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View