Hello dear colleagues
I plan to test the primary antiviral response of CD8 T-cells of different genotypes, WT and with total knockout of some gene. The problem is that the KO mice that I plan to use have defective NK-cells as well, so I`m not quite sure that the effect I`ve got are exactly because of CD8 T-cells but not influenced by defective NK-cells.
So I want to purify CD8 T-cells from the mice of different genotypes and inject them into sub-lethaly irradiated WT mice (550 rad) and infect these mice with LCMV.
The question is: how should I treat these mice? Are there any special peculiarities in their maintenance? Can they survive maintenance in non-SPF conditions for ~8-10 days?
Many thanks!
Irradiated mice Will show mielosuppression according dose of irradiation. Animals can survive in an animal pathogen free environment, Check that other experiments with infectious gents are not present near the place where you keep your animals. Avoid that your animals share cages, food and wáter with other animals in your facility. Good look
Dear Mario Cesar Salinas Carmona, thank you for your response!
Could you tell whether I should add any antibiotic and if yes, then which?
Hi Mario, We would move the mice into new sterile housing after irradiation and give them acidified water to avoid any infection while they are compromised.