Dmytro Shytikov

5 Questions 39 Answers 0 Followers

Questions related from Dmytro Shytikov

How to analyze results from 2 replicate experiments?

Hi everyone! I investigate the result of knockout of a particular gene on the disease severity. I take WT or KO mice, induce disease, and record readings. So, the genotype is the experimental...

03 April 2021 2,819 4 View

How to detect TGFb and IL10 from activated Tregs?

Dear colleagues! I plan to check cytokine production by my WT and KO Tregs following activation, particularly, IL10 and TGFb (TGFb1 or LAP, exactly) as these are reported to be the major...

03 December 2020 9,563 5 View

Why CD4 Treg lose FoxP3 during expansion in vitro?

Hello everyone! I have the following question: I tried to expand CD4 Tregs in vitro to get enough material for WB or any other application. This is my first trial with them. So, I`ve got FoxP3+...

14 September 2020 6,605 6 View

How should I care of mice after sublethal irradiation?

Hello dear colleagues I plan to test the primary antiviral response of CD8 T-cells of different genotypes, WT and with total knockout of some gene. The problem is that the KO mice that I plan to...

23 July 2019 1,948 3 View

CD8 T-cell expansion for adoptive transfer

Hi, guys I`m quite fresh in the field of culturing CD8 T-cells in vitro and I need an advice. I want to test if a population of CD8 T-cells may give protection against LCMV-Armstrong virus...

01 January 1970 6,093 5 View