How Nickel affect biological methane production ?

Soil fertility, plant nutrition

23 December 2020 7,301 5 View

What are the criteria one must look into while searching for a research guide?

As we know that guide plays a vital role in the entire tenure of research leading to PhD, what are the qualities a scholar must look into consideration while selecting a guide?

19 September 2020 2,538 18 View

Mechanism of DNA replication?

I have read this in a book that DNA polymerase can discriminate between ribo and deoxyribonucleoside triphosphate bcoz of steric exclusion of rNTPs from the DNA polymerase active site, as the...

08 June 2020 2,262 6 View

Key elements to consider before rolling out the final survey?

hi, for my quantitative research, i am analysing the pilot survey data. While, my cronbach is looking good, but Tstat, few outer loadings & Pval doesnt look good. Which are the key...

01 May 2020 6,426 10 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

Suggest me the literature related ternary 1D Photonic crystal using TMM method and Matlab Code?

Ternary 1D Photonic crystal using the TMM method and Matlab Code.

17 March 2020 5,970 3 View

Who decides iLref (ref current) for a closed Loop control of Grid Connected inverter?

Power (active, reactive) send to grid depends on iL magnitude and its phase wrt grid voltage. For a closed-loop current control of a VSI (as of now let's consider a single-phase VSI) in a...

14 March 2020 1,810 4 View

What is the best measure of productivity of the faculty/researchers?

There are good number of measures are available to measure the research productivity of the faculty members/scientist like, 1.Scopus "h" Index of the author 2.Google Scholar "h' index of the...

20 February 2020 631 3 View

Which one is best measure of publication quality- SJR or Impact factor of the Journal ?

Some research journals having Impact factor and some having SJR. Which one is the best measure of the quality of reserach paper?

11 February 2020 6,497 3 View

What is G index?

Generally we talk about the h-index of the author or journal. What is G index? is it similar to h-index?, means the productivity measure of the author/researchers. If the author having h-index...

15 January 2020 5,256 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View