How much should be the DNA concentration for sequencing?

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Any one know about pJET1.2 cloning vector with insert gene trouble shooting please?

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Any one can help me about the cloning and colony PCR?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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How to confirm the site-directed mutagenesis result without performing NGS?

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Where should the ARS sequence be introduced on a plasmid?

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Why my colony PCR results of my recombinant bacterial not showing any results?

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How can we now if the promotor in the vector is well?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

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Navneetha Hardikar

100ng/ul is ideal

Lukasz Kuryk

To my view, when you are planning a sequencing, good to have 50-100ng/ul of DNA but this of course depends on the company used.

Oscar L. Sierra

A rule of thumb is 100 ng of plasmid per kilobase pair (1000 bp) of total plasmid size.

Tahera Hashimi

Thanks a lot for your answer everyone.