Hello,

I am doing cloning using the pJET1.2 blunt vector (2.9 kb). The size of inserted gene is around 2.4 kb. When I transformed it into E. coli, I got some colonies and confirmed the positive transforming colony with a specific size by using RE. but when I sent for sequencing, the sequencing result was not okay, during the alignment I got the attention that the gene missed around 100 bp bases from rev

So now confusing what will be the problem, is it problem the length of the gene because almost same to the length of vector? or any suggestion, please?

Thank you in advance