Tahera Hashimi

6 Questions 10 Answers 0 Followers

Questions related from Tahera Hashimi

RNA Extraction Troubleshooting. Anyone familiar with RNA Extraction Using Kit and TRizol Method ?

I am extracting the RNA from E. coli, However, the result in Gel electrophoresis shows quite like degradation even the OD 260/280 and 260/230 are very good (higher than 2. In the gel picture, I...

24 September 2023 9,266 3 View

How to ligate the gene into pET28a plasmid vector?

Anyone can help me how to ligate the gene into the pET28a vector? please write the ligation protocol about buffer and time for ligation that we need. I did but didn't get the positive result from...

09 July 2021 724 8 View

Any one know about pJET1.2 cloning vector with insert gene trouble shooting please?

Hello, I am doing cloning using the pJET1.2 blunt vector (2.9 kb). The size of inserted gene is around 2.4 kb. When I transformed it into E. coli, I got some colonies and confirmed the positive...

09 June 2021 7,420 2 View

Any one know about pJET1.2 cloning vector with insert gene trouble shooting please?

Hello, I am doing cloning using the pJET1.2 blunt vector (2.9 kb). The size of inserted gene is around 2.4 kb. When I transformed it into E. coli, I got some colonies and confirmed the positive...

01 June 2021 3,765 1 View

How much should be the DNA concentration for sequencing?

I did the cloning and extracted the plasmids, now need to send the plasmids for sequencing. is it matter the concentration or no? thanks in advance to help me.

15 April 2021 7,850 4 View

Any one can help me about the cloning and colony PCR?

I am doing gene cloning, the gene was successfully amplified but when I did the transformation, growth some colony in the plate but when did the colony PCR no result at all. I made negative and...

01 March 2021 717 6 View