How much proteins get diluted in BAL fluid compared to that of airway surface liquid in mice when it is lavaged with 2 ml PBS?

02 January 2014 1 9K Report

I am trying to extrapolate airway surface liquid concentrations from BAL concentrations. If anyone can help me with this with appropriate references?

Alok Kumar Soni

this is very important question even for me also. i insert 1.5 mL of PBS in a mice for BALF and i get average protein concentrion is 0.2 to 0.8 mg/mL. since in real time the total volume of surfactant of lungs would be less then 100 uL (cant say exactly) and we collect 1 to 1.5 mL total wash. so i am too waiting for answer of this question

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