Lokesh Sharma

4 Questions 4 Answers 0 Followers

Questions related from Lokesh Sharma

Has anyone measured cathepsin B activity in the cell supernatants or tissue fluids?

We are trying to measure cathepsin B activity in the cell supernatants or broncho-alveolar lavage fluid. Is there a good assay that can measure the activity?

22 October 2021 3,971 1 View

Does anyone know some good quality histone?

I am testing histone toxicity in cells and then planning to test the same in animals. I have total calf thymus histone from santacurz and it kills cells effectively but has very low solubility (...

28 May 2014 789 0 View

ROS assay in RAW 264.7 Cells.

I am trying to work out DCFH-DA ROS assay in RAW 264.7 cells and it is giving me hard time to work it out. I am doing treatment for 24 hours and I tried both preloading and loading DCFH-DA at the...

31 March 2014 3,866 3 View

How much proteins get diluted in BAL fluid compared to that of airway surface liquid in mice when it is lavaged with 2 ml PBS?

I am trying to extrapolate airway surface liquid concentrations from BAL concentrations. If anyone can help me with this with appropriate references?

02 January 2014 9,349 1 View