Hi all,
I am culturing primary microglia and using M-CSF for their expansion. Up until now, we have been producing our own M-CSF from L929 M-CSF O. E. cell line, but due to high production costs and laborious procedure we are considering buying purified M-CSF, also to reduce inter-experiment variability.
I have found conflicting information about the dose of M-CSF to use for primary microglia expansion, and I have little way of knowing how much M-CSF I was using, as it is secreted in the medium and we would use the conditioned medium as it is.
Microglia people, how much purified murine M-CSF do you use in the growth medium for your primary microglia?
Thanks in advance!
I have always got good microglial preps using 100 ng/mL M-CSF and GM-CSF as described in the paper below.
Article Anti-TLR2 antibody triggers oxidative phosphorylation in mic...
Dear Roberta!
Mixed glial cultures were treated with 25 ng/ml M-CSF for 96 hours.
Article M-CSF increases proliferation and phagocytosis while modulat...
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