Roberta Fresia

4 Questions 4 Answers 0 Followers

Questions related from Roberta Fresia

Wisdom of masses for cuprizone handling?

Hi everyone, I will soon start setting up the cuprizone model of demyelination/multiple sclerosis. Cuprizone is purchased from Sigma in powder form and mixed with regular rodent chow (0.2% w/w)....

10 January 2024 4,260 0 View

How much M-CSF for microglia primary culture?

Hi all, I am culturing primary microglia and using M-CSF for their expansion. Up until now, we have been producing our own M-CSF from L929 M-CSF O. E. cell line, but due to high production costs...

08 November 2021 2,213 3 View

MRNA isolation from acutely isolated microglia, tips?

Hi everyone. I am going to isolate microglia from 2 months old mice using the Percoll gradient (30, 37 and 70%), for subsequent FACS sorting and mRNA isolation for RT-qPCR on inflammatory...

02 June 2021 5,414 6 View

Issues with my microglia primary culture?

Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...

07 October 2020 4,082 3 View