Conducting a survey to understand the response to work from home during the covid-19 lockdown?

Greetings, We are conducting a survey on work from home and its impact, please fill the questionnaire and contribute to the growth of academic understanding of the concepts and for policy...

03 May 2020 3,758 22 View

What are recommended temperature conditions for purifying his tagged proteins under denaturing conditions(GuHcl)?

Hello everyone, I'm trying to purify his-tagged protein under denaturing conditions using Guanidine Hydrochloride (Protocol from GE Healthcare-attached below). Though I'm getting my protein in...

10 December 2019 8,654 3 View

How to access stress/ strain at integration points in a umat code?

Im trying to code a damage model for beam elements, for which I know the strain at rupture. I was wondering if I could access the strain at the center of the beam to start and progress damage. As...

28 May 2019 9,755 1 View

Can any one help me to get the MATLAB code for cluster head selection and routing in wireless sensor networks using PSO and GA?

wireless sensor networks- optimization techniques

10 May 2019 2,944 1 View

Bacterial intracellular synthesis of nanoparticles,Could anyone suggest any standard bacteria(preferably gram negative bacteria)for NP synthesis?

Hi, this is part of designing a practicles for students, could anyone suggest any bacteria mediated intracellular synthesise of any nanoparticle (10-50nm) and that gives highly repeatable...

13 December 2016 3,835 4 View

What is the method for separate out the mixture of Ethyl acetate and tetrahydrofuran ?

30 June 2016 9,968 9 View

What are the possible causes of piston or gudgeon pin failure?

Piston pin is subjected to both shear and bending loads. I need some clarity in this scenario to complete my paper. The system runs at a wide range of RPMs , so fatigue also may be one of the...

04 March 2015 4,858 4 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

22 December 2014 2,981 7 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

17 July 2014 5,372 2 View

Is it possible to implement string/text matching using traditional neural networks?

Neural networks known for massive parallelism and for pattern recognition and matching. Based on these, can we use the neural networks for matching strings in large texts. If so, how to convert...

13 May 2014 7,482 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View