Hi, 

In terms of Ab coupling to the Dynabeads M-270 Epoxy coupling of large amounts of Abs (20-30microG Ab/mg beads) is not recommended. If loading 20µg of Abs to the beads, the total amount of Abs covalently coupled to the beads will not be more than 13µg/mg beads. Up to 10µg one will find a linear relationship between added Ab and coupled Ab. Above these concentrations the Abs will precipitate down to the bead surface but a reduction in covalent bonds is seen. Actually, less than 10 µg ensures efficient covalent binding (no leakage) and functionality.

kind regards

ketil

Hi

Feel free to contact me at [email protected] for discussion as I am working as a staff scientist in R&D at the production facility for the Dynabeads in Oslo, Norway.

kind regards

ketil

Hi, 

In terms of Ab coupling to the Dynabeads M-270 Epoxy coupling of large amounts of Abs (20-30microG Ab/mg beads) is not recommended. If loading 20µg of Abs to the beads, the total amount of Abs covalently coupled to the beads will not be more than 13µg/mg beads. Up to 10µg one will find a linear relationship between added Ab and coupled Ab. Above these concentrations the Abs will precipitate down to the bead surface but a reduction in covalent bonds is seen. Actually, less than 10 µg ensures efficient covalent binding (no leakage) and functionality.

kind regards

ketil

see ref Alber et al., (2007) Determining the architectures of macromolecular assemblies Nature 450, 683-694 and Oeffinger et al. Nat Methods. 2007 Nov;4(11):951-6. Epub 2007 Oct 7 for very nice co-IP work.