Hello Nisha Gautam

You can use 0.01 to 1mg/ml DNase I. For each cell type, the working concentration must be determined individually. For optimal enzyme activity, DNase I requires divalent metals. So, you need to add 5 mM MgCl2.

Best.

@Malcolme Nobre

Thanks for the reply. I qas searching in the lab “. So i found Benzonase2.5 KU instead of DNase1 . Can tpu please suggest how much benzonase to be used from the atock for the one prep.