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Questions related from Nisha Gautam
Hello Everyone! Recently i tried Mito protein western blot from whole lysate of mice tissue for one of the subunit of COX 8A (Size= 8KD) and One subunit of NADH dehydrogenase (SIZE = 17KD)...
15 December 2022 8,839 1 View
Hello everyone! I have RNA seq dataset for two groups knockout and wild types of mice samples. I have the normalized values in terms of quant all datasets. Please guide me how to perform PCA on...
22 June 2022 6,309 4 View
I have population data set that i need to split into two papers. But initial tables like patients and healthy individuals info is same /common. I have already published that info with one paper....
19 May 2022 9,392 5 View
I have received RNA seq data from three KO mice and wild type mice. The mice are littermates from commom father and two mother and age of 2-month old. I got the RPKM values and performed GSEA...
22 February 2022 7,445 1 View
I have to standardize histone purification from sciatic nerve of mice with RIPA buffer. Can you please suggest me how much DNase to be used in RIPA buffer for protein isolation generally. Thanks
04 January 2022 2,176 2 View
I am doing Multiplex PCR, which is already standardized at annealing temperature 55 C. I am running mice DNA in PCR. I got good amplification of one sample which is control (Original DNA...
30 November 2021 3,771 5 View
