Hello, I would like to re-probe my Southern blot membrane, I'm following the chromogenic detection with NBT/BCIP and the protocol calls for Dimethylformamide but it does not say anything about the volume or concentration to use, nor I can find a protocol using dimethylformamide for stripping a nitrocellulose membrane.
Do you know a protocol I can follow for this?
Hi Barbara, this discussion might help you;
https://www.researchgate.net/post/Does-anyone-know-if-formamide-can-be-used-instead-of-dimethylformamide-to-strip-nylon-membranes-from-Southern-blot
Regards....
Thank you Jawwad Ahmad I read that discussion and although Uwe does mention the use of formamide, this is for stripping Southern Blots with a different detection method.
I'm using NBT/BCIP and DMF is required for clean-off the membrane of the NBT/BCIP substrate, so as mentioned by @Artur Burzynski formamide won't be useful :/
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