Glycerol layer is automatically separated after the complete process of biodiesel preparation. Traces of glycerol can be separated by simple centrifuge method.
Further you can wash biodiesel sample with D/W to remove the traces of glycerol , add anhydrous Na2SO4 to remove water traces from biodiesel and filter it.
All the above answers are self explanatory. Biodiesel is produced by transesterification of oils ( Vegetative oils, animal fats or oils derived from algae and microbes). After the above process the biodiesel and glycerol are separated, biodiesel in the top layer and glycerol in the bottom layer. After the layer separation the biodiesel if further purified by washing with distilled water and can be dried also.
Do you have a new process of separation of two layers by electrostatic separation?