Follow the intructions, it´s the true amount of ab.

The surface of the beads  is covered with protein A/G like a cubic best packing micell

Martin

Thank you! Could you add your protocol, if you have one, so that i can compare it with mine? Thank you!

http://www.piercenet.com/product/protein-ag-magnetic-beads

The data sheet should tell you the binding capacity of the beads - work out how many ug of antibody/protein will bind to the ul of beads you are using; then add a slight excess. That should be more than enough. 150 ug for 250 ul beads seems a bit excessive in my opinion!

Thank you Vikram, useful suggestion!