How much ampicillin should I add in my LB medium (250ul) ?

15 February 2023 1 7K Report

I used 125ul of Ampicillin stock (x1000) to make my agar plates

The working concentration of ampicillin is 100 ug/mL. It's typical to have your stock at 100 mg/mL (x1000) and then for example, to 100 mL LB add 100 uL of stock ampicillin, thereby resulting in a final concentration of 100 ug/mL.

If your LB was 250 mL, you'd need to add 250 uL of your 1000x stock.

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