How much of AM inoculum is added in Inoculation work in Pot experiment?
I am trying to use Legumes and grasses species in green house experiment? not sure which are the best legumes and grasses species to carry out work with AM species? If any body can help me in this matter? After the propagation of AM fungal species, how much of Inoculum of Fungi could be used to carry out inoculation work? I am planning to use Glomus mosseae and claroideoglomus claroideum as single inoculation and mixture of AM both species? which grass species and legumes species are best in this combination.Look forward for your answer.Thank you.
About the use of single or mixed inoculum, it depends on the information you want obtain: if you look for the effects induced by a given fungus, on a plant chosen as model system for basic research, then the single inoculum is better. If you look for effects on plant growth for applicative purposes, generally a mixed inoculum gives better performance. About the quantity, it depends on the characteristic of the inoculum used.
Medic (Medicago truncatulata) and leek (Allium porum) are standard highly mycotrophic host plants used in greenhouse to produce AMF inoculum. Root fragments colonized with the desired AM strain and mature spores are the best source of inoculum. You don't need much materiel for starting a new culture. Just be patient since it takes two - three months to get a significant amount of spores in pot culture following inoculation.
Thank You very much Franck Stefani and Graziella Berta. I have used Sorghum seeds to propagate AM fungal species. I hope it can work. After multiplication of AM culture in pot. I will use pot culture as a source of my inoculum in the experiment, but I want to know which grass species and leguems are best to check mycorrhizal diversity? As we know that AM species may have different contribution to the seedlings. I want to check mycorrhizal diversity in green house experiments. If any one can help me in this matter. Thanks.
Dear Shakoor, you can follow the advises of Franck Stefani and Graziella Berta. You can use Maiz , sesbania, cassia tora for inoculum production. Usually grasses and legumes growing in natural conditions are mycorrhiza. You can check the grasses and legumes in your surroundings and select the best one for diversity test.
You should visit the INVAM website for more information:
http://invam.wvu.edu/methods/cultures/single-species-cultures
An excellent website for inoculum production and species identification. It is sufficient for you, Mr. Shakoor.
Most legumes are highly mycorrhizal, but not lupins which are notably non-AM.
The paper by Wang and Qiu provides a nice list of AM and non-AM plant species,
http://mycorrhiza.ag.utk.edu/reviews/rev_wang1.pdf
Dear Erik Verbruggen Thank you very much for such a useful information.
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