I used 0,65-0,7% Phytoagar (Duchefa). 0,7 is suitable für hairy roots, for leaf explants ist es better to unse a slightly smaller conentration that results in a very soft medium.seforit is
it's upon to degree of solidity, based on my experience, in MS medium (pH= 5.7) less than 6 gr/liter results in semi solid, so u can use 6 gr or less
The amount of agar to be used as a solidifying agent varies between 0.4-0.8% . i am using .8% in tomato and getting good results as well
Depending on agar brand, concentrations between 0.5-1% are generally used for this purpose.
Dear Saiful, there're many factors that determine the concentrations of agar needed. As Seied mentioned, the brand is among the most important factor. Other colleagues have also mentioned the goal of your work: proliferation, rooting.... The species is also important. For extra details, read this pioner and valuable paper " Debergh, P. C. (1983). Effects of agar brand and concentration on the tissue culture medium. Physiologia Plantarum, 59(2), 270-276."
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