How many times is infection by Erwinia sp. recommended, in Vicia faba, before measuring the infection in the phloem in the plant ?

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Praveen Roylawar

you can measure infection more precisely by q-RT PCR absolute quantification of fungal DNA in phloem of plant material

Nilmara Pereira Caires

i would like know if you are sure that Erwinia infects the phloem of beans. because I'm conducting studies in eucalyptus ( E. psidii) and not found the bacteria in the phloem tissues. I'm sure that in this host, it is restricted to the xylem.