How can I calculate the MDA concentration in leaves (100 mg) after taking absorbance at 532 nm and 600 nm?

100 mg tomato leaf tissue from a sample was ground in liquid nitrogen homogenized in 1 ml of 5% TCA and centrifuged at 8000 rpm for 30 min at 4 C. One ml of supernatant was incubated with 4 ml of...

12 May 2014 850 10 View