Mohd Najib Mostafa

10 Questions 12 Answers 0 Followers

Questions related from Mohd Najib Mostafa

How many times can we immunize mice before hybridoma preparation?

Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...

10 January 2021 9,716 3 View

PADI2 Cell Line?

Dear Researchers, Do you know of any PADI2 expressing cell lines? It must be either neuronal, astrocyte, or any cell that is usually found in Brains. Thank you.

11 August 2020 1,997 2 View

How to Preserve Murine splenocytes?

Dear Researchers, I would like to know if there is a method to preserve murine splenocytes without staining for future FACS analysis for immunophenotyping? Thanks in Advance. Mohd Najib Mostafa

03 March 2020 568 2 View

Need suggestions for Murine T Cell subset detection FACS ?

Dear Researchers, I would like to know if it is ideal to detect murine T cell subsets from the spleen with the following markers? TH1: CD3+ CD4+ CXCR3+ TH2: CD3+ CD4+ CXCR4+ TH17: CD3+ CD4+...

24 February 2020 7,013 3 View

How to perform FACS analysis using Mice lymphoid organs and blood?

I am a new learner of FACS analysis and will be needing this method for immunophenotyping, i.e to detect certain immune cells. I would like the help of experts to help me with certain procedures...

04 April 2019 5,838 1 View

How to perform Western Blot detection of low molecular weight proteins?

I am performing experiment with C6 glioma cells which are known to express S100B usually according to ATCC data sheet. But when I perform western blot with the cell lysates, I fail to detect any...

29 March 2019 5,777 8 View

Samples having tails while stacking during electrophoresis

I was seeking suggestions regarding a problem I am having with my western blot. During the stacking phase my samples have tails behind which i guess is leading to smeared bands when i observe the...

01 January 1970 3,308 11 View

Any published gating strategies for analyzing Murine T and B cell population in peripheral blood?

Dear All, I am wondering if you know of any gating strategies for analyzing T and B cell populations in Murine blood samples collected in heparin-coated tubes. As far as I know, 100ul of blood,...

01 January 1970 6,563 3 View

Cerebellar Organotypic Slice culture Not flatenning?

Does anyone experience Cerebellar slice culture to not flatten even after 12 days post culture? I would like to know why and how to solve the issue? Thanks.

01 January 1970 6,977 0 View

Any Expert on BD FACS Calibur?

Hello, I have some queries regarding the use of FACS Calibur and data analysis with FLOWJO. Is there anyone who can help me out? Thanks in Advance.

01 January 1970 7,519 1 View