8 Questions 11 Answers 0 Followers
Questions related from Mohd Najib Mostafa
Dear all, Recently I have been working with monoclonal antibody production and I usually Immunize mice for a total of 4 times with the last one being diluted in PBS 3days before fusion...
10 January 2021 9,543 3 View
Dear Researchers, Do you know of any PADI2 expressing cell lines? It must be either neuronal, astrocyte, or any cell that is usually found in Brains. Thank you.
11 August 2020 1,860 2 View
Dear Researchers, I would like to know if there is a method to preserve murine splenocytes without staining for future FACS analysis for immunophenotyping? Thanks in Advance. Mohd Najib Mostafa
03 March 2020 500 2 View
I am a new learner of FACS analysis and will be needing this method for immunophenotyping, i.e to detect certain immune cells. I would like the help of experts to help me with certain procedures...
04 April 2019 5,778 1 View
I am performing experiment with C6 glioma cells which are known to express S100B usually according to ATCC data sheet. But when I perform western blot with the cell lysates, I fail to detect any...
29 March 2019 5,706 8 View
I was seeking suggestions regarding a problem I am having with my western blot. During the stacking phase my samples have tails behind which i guess is leading to smeared bands when i observe the...
01 January 1970 3,254 11 View
Dear All, I am wondering if you know of any gating strategies for analyzing T and B cell populations in Murine blood samples collected in heparin-coated tubes. As far as I know, 100ul of blood,...
01 January 1970 6,509 3 View
Does anyone experience Cerebellar slice culture to not flatten even after 12 days post culture? I would like to know why and how to solve the issue? Thanks.
01 January 1970 6,927 0 View
