isolation of plasma cell from mice spleen to produce monoclonal Ab invitro
Thank you polina weitzenfeld. but i do not have full access to this web
Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...
17 February 2021 2,141 9 View
Dear Connections, I hope you are doing well when I made an Autotune on GC-MS to check the apparatus, it does not continue the process, and there a problem in the mass Filter Electronics as shown...
10 October 2020 8,131 3 View
I am measuring the drug release of a few drug-eluting implants in dissolution medium in different time intervals. All the measurements are in triplicates. Would ANOVA or TTEST is an appropriate...
20 September 2020 8,183 5 View
Good afternoon everyone, I hope you are doing well I had several samples of liquid detergent I wanna to soluble it with a solvent. I tried with both of ( water, Ethyl alc., Parrafin Oil, DPG,...
05 August 2020 8,664 3 View
Hi everyone, I hope you are well and stay safe. I am an operator for GC-MS instrumental and my company replace the column with another new one which called DB-WAX (60m*0.250mm*0.25microm). A...
14 June 2020 4,440 3 View
Under the conditions of the Corona virus, most countries tended to use the method of distance education, using various methods, including: e-learning platforms, and social media. It is true that...
10 April 2020 7,181 3 View
I wanna to injection sample which contain only ethyl alcohol is that okay or not?
06 April 2020 9,164 4 View
We just got a new freeze dryer (Biobase) but unfortunately the machine arrived without pump oil, so could any machine/ car oil be used as a substitute? if not could you pls suggest any other type...
06 February 2020 167 3 View
I am an associate professor, I am suffering from a lack of expert persons under the title: "Full professor" in the field of antibacterial potential of medicinal plants. I should be most grateful...
28 December 2019 9,919 5 View
I work the research about stabilization of soil by Biological soil crust especially algae (Cyanobacteria and green algae) Could anyone please help me about this topic soil stabilization using...
17 December 2019 8,682 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
28 February 2021 2,534 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View