isolation of plasma cell from mice spleen to produce monoclonal Ab invitro
Thank you polina weitzenfeld. but i do not have full access to this web
Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...
28 July 2024 2,136 4 View
I use MATLAB to define SAP Model using API function but when trying to define time history direct integration case, the non-linear parameters change from the default which affect the results and...
04 June 2024 2,369 1 View
I want to publish my thesis research, so could you please explain the process in detail. Many thanks for your support in advance. Best regards, Mohammed Alhamati
10 April 2024 6,098 2 View
I have aqueous and methanol (or ethanol or ethyl acetate) extracts from some local plants and am seeking a research collaborator. I will provide dry crude samples for phytochemical analysis and...
10 February 2024 2,761 0 View
Hello guys One of my articles has been accepted in one of the journals of Pagepress publication. How can I get a full waiver from this publication? I explained to the managing editor of the...
04 February 2024 3,375 2 View
I want to collect field plant species data for several plots and sites. Which Android app is suitable for this task? I have the species list in an Excel file. Please recommend an app that you used...
04 February 2024 5,232 0 View
I am a PhD student, and my thesis is on supply chain resilience. My model includes four independent variables, one moderator, and one dependent variable. The moderation in my model represents my...
22 December 2023 4,962 8 View
Integral transforms play an important role in cybersecurity. Complex Integral transforms are used in security encryption, decrypting information and data, analyzing data, and predicting threats.
28 November 2023 4,844 4 View
I am doing my PhD thesis, and moderation is the main contribution (novelty) of my study. However, the study findings revealed non-significant moderation. R2 with moderation is 69%, while R2...
27 November 2023 7,681 7 View
The answer is the complexity of the conversion kernel, which is of great importance in real-life applications, most importantly in encrypting images and videos, and it has been applied practically.
27 November 2023 622 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View