Please help me. I conducted a cell proliferation experiment, continuously diluted threefold from 10000nM to 1.5nM, and processed the data using Prism. Why is it that the fitted IC50 value cannot be expressed as 123.74nM, but 123nM is allowed?
Thank you so much.
The difference between expressing the IC50 value as 123.74nM and 123nM lies in the significant figures. When reporting values, it's important to consider the number of significant figures that accurately represent the precision of the measurement.
In scientific notation, the significant figures are the digits that carry meaning in a number. When writing the IC50 value as 123.74nM, it implies that the measurement is precise to the hundredth place (two decimal places). However, when rounded to 123nM, it simplifies the value to three significant figures, which is appropriate if the precision of the measurement does not warrant more detailed reporting.
Prism may automatically round values based on significant figures to simplify and standardize reporting. In this case, rounding to 123nM may be a result of Prism's default rounding rules for significant figures.
Hello Mu Qinyao It seems that your answer lies in this option of the software.
Show this many significant digits (for everything except P values)
I have sent you its image.
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