A few of my co-researchers have mentioned that it is good to have more than one stop codon at the end of the protein, just to make sure that translation stops. Now, do we need more than 1?
One of the researchers also suggested to add 3 stop codons in 3 different reading frames. Is it logical to add them in tandem or in different reading frames?
I feel one stop codon is enough to do the job. Thats how the system uses. I don't think increasing the no. of stop codons certainly help in stopping translation.
One stop codon should be enough, if you have a shift in the reading frame you are going to have premature stop codon and/or multiple stop codons. Be sure to have a strong signal of the finish of transcription, in the commercial plasmids you are going to find this for sure. Best, A.
Just one is enough, however, it does not make any difference if you have much more.
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