What is special about the Number 396?

Hey there, i just edited some random empiric dataset and i saw that the number 396 induces interesting percent-outcomes. I set 396 as 100% and every other number - thats the observation - leads...

31 December 2017 7,410 1 View

Plants as Methane Producers? What is the latest standard of knowledge?

Dear All, not only in all known to me textbooks, but also in my academic environment, plants are only discussed as carbon sinks and therefore planting forests is said to be one of the main...

10 November 2017 8,856 8 View

Does Occam's razor favor MACHOs as candidates for Dark Matter?

Dear All, not only in our university, but almost in all well known to me lectures about Dark Matter, MACHOs (Massive Astrophysical Compact Halo Objects) are rather incidentally discussed while...

09 October 2017 6,887 4 View

Multiverse as a inevitably consequence of space expansion faster than light?

Our knowledge from direct observations about far away regions of the universe is limited due to the observation horizon. If the Universe expands and the acceleration gets faster and faster, isn't...

07 August 2017 7,340 0 View

Heisenberg's Uncertainity VS Laplace's Demon ?

01 February 2017 1,464 23 View

Why does Gravity not lead to a collapse of the wave function in the double slit experiment?

Dear people, if a "measurement" is ment to be an interaction of an elementary particle, why can we still see wave-interference patterns in the double-slit-experiment which are conducted in rooms...

01 February 2017 605 18 View

Why is Free Fall universal?

31 December 2016 6,910 1 View

Black Holes as Dark Matter?

31 December 2016 4,813 2 View

Is a perfect simulation of reality impossible because the mass of information creates an insight horizon?

Black holes cause event horizons, depending on the mass compressed into a narrow space (point?). From this analogy, could the quantity (mass?) of information in a narrow space lead to an insight...

01 January 1970 1,352 11 View

Space Curvature - Is there a mathematical description of the fourth drawing?

Dear all, in accordance with Friedmann-Lemaitre-Equation there are three different possibilities of space curvature which can be described mathematically and imparted graphically or analogously...

01 January 1970 690 12 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View