In adults a 'normal' result should be in the range of ... Absolute: 0.2 - 0.8 x 10^9 / L.

mean of 0.5 = 500,000,000.

So per ml = /1000

=~500,000

Hi Philip is this what a healthy donor should have per ml of blood or what you can actually isolate from 1 ml of blood ? I routinely isolate primary monocytes from whole blood using a previously established lab protocol but I can usually only manage to isolate ~2 million from 20 ml's of blood but would love to know of other methods that might give those sort of yields :).

Hi Justin, I think you are right. Since monocytes represents only 10 percent of the PBMCs and per 1 ml of blood (ideally) 1 million PBMCs can be isolated.

As philip you have 500.000 in a 1 ml blood but you can not separate all of it .if you need more you must stimulate donor with M.CSF and you can read Effect of granulocyte-monocyte colony-stimulating factor therathis py on leukocyte function and clearance of serious infection in nonneutropenic patients *.(clinical investigations in critical care)

Article from: Chest | June 1, 2005 | Rosenbloom, Alan J.; Linden, Peter K.; Dorrance, Adrienne; Penkosky, Nicole; Cohen-Melamed, Mark H.; Pinsky, Michael R. | Copyright

I think Justin is right.  Actually,  from 10ml blood, PBMC yield is just 10 million.

Hi we use EasySep monocyte direct isolation kit (magnet based, negative selection) and get around 4x10^5 cells/ml. The purity is claimed >90% based on manufacturer and publication.

Xi is right. magnet based negative selection is very efficient.