Amit Kumar

6 Questions 11 Answers 0 Followers

Questions related from Amit Kumar

How can I extract the sequences of guide RNAs (gRNAs) targeting a specific gene from the DepMap portal?

Dear All, I am new to working with the DepMap database and would appreciate your guidance. I understand that DepMap includes a collection of genes tested for knockdown using CRISPR/RNAi...

27 January 2025 5,287 1 View

When we perform statistical analysis on qPCR data, do we use fold change or ΔCt?

21 August 2024 355 8 View

Can a particular protein dephosphorylated ( at the same kind of residue for example pTyrosine) by two different phosphatases in the same cell type?

Any published example would be a great help. Thanks Amit

23 February 2022 2,206 2 View

Why does SDS solution (10 or 20%) purchased from the company not precipitate?

Sodium dodecyl sulphate (SDS) solution prepared by me generally begins to precipitate in few days. In order to use it every time I have to reuse it. However, SDS purchased from the company...

05 April 2014 4,922 1 View

How many monocytes can be isolated from 1 ml of blood from a healthy donor?

Monocytes per 1 ml of blood.

08 March 2014 8,773 7 View

What are the differences among TB40E, TB40F and AD169 strains of HCMV?

I am looking for specific protocols to culture HCMV strains (TB40E, TB40F and AD169). Can I make viral stocks of TB40E and TB40F in fibroblast cell lines? What makes TB40E and TB40F distinct from...

30 October 2013 9,403 7 View