Hi everyone !
I am trying to extract RNA on drosophila wing discs using the Qiagen RNeasy micro kit but I have contaminants (260/230 ratio
Products / Services Groceries Fancy items Garments Gifts Electronic Goods and Accessories Household Supplies Skin Care Products Fitness or Wellness Products Jewellery Hotel...
20 May 2024 6,387 0 View
Besides the procedures in adapting a psychological scale, are there any prohibitions or limitations in adapting a scale into another language and culture?
29 February 2024 8,875 3 View
Currently, I am adapting the Workforce Agility scale adapted to Indonesian language and culture. To provide further clarification for items, I am adding contextual information within parentheses...
29 February 2024 5,159 3 View
Cuáles son las causas y consecuencias del involucramiento parental?
13 December 2023 1,606 0 View
Hello, Could anyone direct me to a consultant specialized on developing disso method for QC and IVIV biowaiver please? Does anyone could direct me to disso training covering these two topics...
28 September 2023 2,994 1 View
Hi everyone, I'm trying to estimate the probability of having a possible diagnosis X (presence vs absence) according to age (continuous variable), gender (3 modalities), and nationality (157...
29 August 2023 1,202 0 View
Hi Everyone, I am using the software COLONY to make sibship reconstruction with noninvasive samples (recovered on the field => hairs, feces; using co-dominant microsatellite markers) of Sus...
07 February 2022 1,431 0 View
Hi folks, I want to perform ATAC-seq (a chromatin conformation measuring technique) on drosophila wing imaginal discs. Can you recommend a protocol to isolate intact nuclei ? I tried Buenrostro...
09 April 2019 7,342 6 View
I would like to perform some Melt Flow Index (MFI) measurements on PA and ABS polymers. I read the ISO 1133 and ASTM D1238 standard where it is written that hygroscopic materials (such as ABS or...
07 February 2019 2,833 4 View
Hello everyone, We are trying to perform laser microdissection on human muscle. We prepared our 15µm slides of muscle on PEN slides and performed IF and counterstained the slide with dapi (we...
03 September 2018 7,610 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
How can parents ensure their drinking water is free from harmful contaminants?
27 July 2024 8,722 6 View
I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...
24 July 2024 9,688 0 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View