I am afraid that my RNA degrades. I have prepared about 1.5 months back and will send for sequencing in the first week of Jan. In total, about 2 months. I think it wont degrade but I am afraid..it is a precious sample and tough preparation.
Amit Kumar
I have not done snap frozen but I keep just after RNA preparation in -80 and checked bioanalyser when I was storing sample. since then I have not taken sample out and I will have to wait till 10th Jan as it is Christmas time.
and did you do transcriptomics with the stored sample or qPCR?
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Abhijit Maji
If you store with RNA storage solution from Thermo Scientific in -80 it will stable for 6-12 months but better to synthesize cDNA and store it in -20, because RNA has its own self life.
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