How many Baculovirus copies can one insect cell (eg SF9) produce in 48 to 72 hrs after infection?

13 January 2016 1 9K Report

Dear all, Does anyone happen to know the copy number of Baculovirus each infected cell can produce after 2-3 days post infection in normal conditions? Thank you.

Konrad Miatkowski

It varies from virus to virus and from culture to culture. But is somewhere between 10 and 100 pfu/cell . Most o the time I was getting 50-75 pfu/cell range.

For example, sf9 cell culture infected at 3-4e6 vc/ml would generate a virus stock at +/- 1-3e8 pfu/ml.

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