How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

What are the first steps to learn to write meta-analysis ???

any suggestions for beginners ???

31 December 2020 3,055 4 View

How can we guide students towards active learning? What are the activities for such practices?

I am asking this in relation to practice in active learning in physics education in context of Nepal's education system where we highly focus on content based and rote learning.

02 December 2020 6,686 3 View

How to create a index using principal component analysis?

Hi All I need your guidance on how we can combine multiple variables and develop one index. The goal is to have a index based of which we can plan for staffing. The variables are: daily patient...

21 October 2020 422 6 View

How to remove abnormal behavior at support of FE model of RCC Beam?

Hi, I am modelling a half model of RCC beam in ANSYS Mechanical APDL. The model I have created is exhibiting abnormal behavior at the support with abnormal cracks. I would be grateful if you...

12 October 2020 8,596 3 View

How are Spatial Cognition and cartography interrelated? What are their major uses and functions in field of cartography?

Major details about their interrelations, their major uses and functions.

22 September 2020 9,796 4 View

How to effectively extract DNA from dairy cattle's milk?

Does anyone have experience of extracting DNA out of dairy cow's milk? I am interested in the DNA present in the B-cells. I have done extraction from blood but want to try extraction from milk. I...

02 September 2020 3,536 5 View

How to calibrate the parameters of coupled damage-plasticity microplane model of concrete for FE modelling of RCC beam in ANSYS Mechanical APDL?

Hi, I am trying to model an RCC beam in ANSYS Mechanical APDL. I have the value of Compressive strength, and modulus of elasticity of the concrete of the experiment I am trying to validate ? Can...

28 August 2020 4,623 2 View

In what concentration should i preserve bovine PBMC in 10% DMSO 90% HS for B-cell count via flow cytometry later on?

I want to preserve bovine PBMCs for doing flow cytometry later on. It doesn't seem feasible to count PBMC cells for preservation as there will be lots of samples. Is resuspending the PBMC pellets...

07 July 2020 8,228 2 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

What are difference between laser diodes, simple light emitting diodes and power light emitting diodes when operated in the visible spectrum ?

I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...

22 February 2021 4,240 4 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

How do I plot Pressure against crank angle degree in Fluent?

18 February 2021 8,390 3 View