How long will the spore of U. virens (false smut) remain viable under normal freezing or in room (temp. varying from 28-32 degree Celsius)?

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How to calculate SNIR in presence of Beamforming?

I would like to solve beamforming weight in a C-RAN environment for a reinforcement learning task. I have studied different beamforming weigh calculations such as Capon and MUISC. But, I still can...

17 May 2024 7,325 1 View

XRD peak deviation?

In XRD analysis, when we move to a higher angle, like above 50 degrees, if I have two reference lines of different phases, one matches the peak and the other is towards the right of the peak with...

31 March 2024 1,812 2 View

Will the temperature of PCR lid affect the overnight ligation at 16 degree celsius?

I had set up overnight ligation reaction at 16 degree celsius in a PCR machine but the lid temperature was high around 95 degrees. Will it affect the efficeincy of ligation if done in PCR in this way?

22 January 2024 2,587 2 View

Is it possible for yttrium or yttrium oxide to react at 760 degree Celsius during casting of magnesium, if its added to reinforce the magnesium?

Casting was done under SF6-CO2 and argon gases environment.

02 December 2023 4,920 2 View

Is there any non kit based method to isolate PCR amplified product?

Is there any manual method to precipitate PCR amplified product?

01 December 2023 5,539 4 View

For doing blunt end cloning shall I dephosphorylate the linearized vector before blunting?

To ligate blunt insert into blunt vector does the vector need to be dephosphorylated at the time of ligation? I have digested my vector with restriction enzyme that create sticky ends that...

15 November 2023 448 0 View

Will the gene under EF1a promoter (from mammalian expression vector) be expressed in any bacterial host?

I have inserted a gene in downstream to Ef1a promoter of my mammalian expression vector. Can I check the protein expression using any bacterial expression host? Or, will it only express in any...

06 November 2023 3,410 4 View

What are the odds that, the amplicon generated using primers carrying overhangs will have blunt end and does not carry any overhangs ?

The DNA polymerase fills in the gap due to overhangs (using a pair of primers carrying overhangs) during the 2nd round of replication in PCR. Does those gaps actually get filled or they have...

02 November 2023 6,400 1 View

How can I ligate an insert digested with same restriction enzyme from both end to a vector digested with same enzyme?

I have insert having flanking regions with XbaI site and and a vector digested with XbaI. How can I prevent self ligation in insert as well as vector before ligation?

31 October 2023 8,865 1 View

How can we develop robust & scalable strategies for precise gene editing in pluripotent stem cells, allowing us to correct disease-causing mutation ?

Even though we have advanced so much into using Genome with Technology to cure diseases, still newborn babies are suffering of abnormal genetic mutations. How can we scale these methods to make...

12 June 2023 6,781 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View

Can I separate xylene from paraffin after deparaffinization of microtome samples and reuse the xylene?

...

24 July 2024 1,559 1 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Please inform me about the International Conference on Plant Biology?

Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.

14 July 2024 3,120 7 View