It is known that as cells divide, transfected plasmids are diluted among the cells but is it known how long is the half-life of a plasmid (but not the protein expressed from that plasmid) in mammalian cells?
I am not asking about the cytosolic turnover of a plasmid, but I am asking how long is the half-life of a plasmid in mammalian cells? It seems that a plasmid in cytosol degraded soon but we see expressed proteins for a long time, which means that plasmids are not degraded for a long time in the nucleus.
Many thanks,
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This strongly depends on th structure and character of your plasmid (shorter sequence (vector in total) in generally more stable than long plasmids) an well on the cell type used in your experiments. Also see the aforementioned answer/proposed literature and I would propose to consult futher literature (see PubMed).
Dear Dr. Rasulov,
It has been estimated that the half-life of naked plasmid DNA in the cytoplasm of cells ranges between 50 minutes and 5 hours.
This is a good question.
Best Wishes!
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