Does anyone know how long is IWP-2 (inhibitor of Wnt secretion) stable at 37°C?
Pupil Core for simultanious recording of a child and a mum and is willing to share experiences?
07 July 2024 9,066 0 View
Hello, what THP-1 differential markers do you use in the Western-blot method? I am looking for proteins that are characteristic of M0, M1 and M2. Do you have something proven and reliable? Greetings
24 June 2024 9,025 0 View
I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...
04 June 2024 6,924 0 View
For several years now, the procedure of granting a vote of confidence to the municipal executive by the legislative has been in force in Poland. It takes place annually and is not related to the...
16 February 2024 4,769 2 View
I completed the experiment for one of our colaborators (I measured inhibition of an enzyme by 3 compounds). Unfortunatelly compounds were not active in the range of dilutions that I had used (up...
06 February 2024 3,542 2 View
Climate change is one of the most serious challenges humanity has ever faced. To address the problem UN Framework Climate Change Convention was established Climate change education has been a...
07 September 2023 2,621 2 View
I have the lite version of ZEN ZEISS 3.7 software and I can't use the "Image Analysis" option because it is only available in the blue version. Is there another way to count the number of...
26 July 2023 7,426 1 View
Dear Colleagues, which software is in your experience most suitable for analysing semi-structured interviews? Can you give examples of articles where such data were analysed and results...
14 December 2022 9,735 4 View
Hello RG, I have results from Sanger sequencing for validation of multiple variants I have found in my patients cohort, can you please instruct me if there is some sort of open source program...
21 August 2022 1,286 0 View
Dear fellow Researchers, I have a question - can anybody explain, show literature sources or in any other way guide our team in the complex topic of mechanisms of uptake of organic contaminants...
08 August 2022 4,974 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View