Hey guys,
I want to prepare Illumina NGS libraries in our lab without a kit. My plan is to prepare the libraries and pool them in the next 4 weeks. Then I am on vacation for 2 weeks and want to run the samples after my vacation. My friend told me that the libraries are not so stable that they will run properly. So my question is:
How long can I store libraries (frozen) until they will be degraded?
It would be great to get a fast answer as I want to start this week. Thanks a lot.
Julia
We have kept them cold for many weeks without issue. Our sequencing facility has also re-sequenced a library for us after 6-8 weeks and it worked fine. These were complete libraries though (not some intermediate stage), and post size-selection and purification.
Thank you for the fast answer. This makes it quite easy to plan my project.
We only keep our libraries 6 weeks, and it works fine. I wouldn´t try going longer.
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