N-(p-Isothiocyanatophenethyl)spiperone HCl is a neurotoxin that targets 5HT2A and D2 receptors and I want to avoid repeated weighing by making up and aliquoting a 10 mM stock solution. Will my aliquots be stable over several months?
The aliquoting good for use upto 6 month and avoid the thaw and freezing.
make aliquot and store -20 C.
Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....
03 March 2021 506 3 View
Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
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I want to know how to change the status of an article.
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I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...
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Hello, we are currently working on absolute quantification with SYBR Green qPCR. We excised from a plasmid containing the cDNA, a fragment, which will be amplified by the primers we used in our...
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I am looking for a reliable and sensitive AMH ELISA for use with rat serum (and potentially plasma). Recommendations are welcomed! Thank you in advance.
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" Picture a world where every product and appliance is environmentally friendly, where every supermarket item is fair trade, where corruption is an urban myth and poverty a long-distant memory....
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Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...
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I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...
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Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
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Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...
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While surface roughness is an obvious factor, this is not what is exactly asked. Rather, periodic variation of exchange current density (of an electrode) with solution chemistry (of electrolyte)...
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I conducted a study to investigate stock market reaction to equity issues announcement and i ended up with 64 announcements and i then used the announcement day returns as my DV and investigated...
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I wanted to use commercial Giemsa staining solution and diluted it 1:10 in pH 6.8 Weise buffer. It says to filter the solution, however, most of the stain seems to preticipate in the filter. The...
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I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...
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One of the properties of complex adaptive systems is inherent order. It means that system can be orderly even without central control. According to Prigogine,1967, Holland,1998, and Kaufman,1995.,...
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The research focuses on the mangrove forest ecosystem. Then it will conduct biomass and carbon stock estimation at the plot scale? How to scale up forest carbon stock assessment from plot scale to...
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