I would like to know how long does it take for surface protein expression after treating the cells with growth hormone or any other peptides if you perform western blott or immuno histochemistry.
Can you see the short term effect immediately after treating the cells with GH (growth factors) in 2 hr or 3 hrs.. or the long term treatment for 24 or 48 or 72 hrs have more pronounced surface expression with these treatments?
Also is it the same case with the peptide hormones?
When i look at the effector proteins such as PKA/PKB i can correlate the effect through Western blott in few hours per say 2 hr with the Growth factors, but the surface protein expression took 24 or 48 hrs can anyone of you have observed something similar?
It all depends on the cell type and the proteins being monitored. In general the intracellular signaling pathways are rapidly activated by protein/peptide hormones while the proteins requiring DNA transcription take somewhat longer.
Cells that express prepackaged proteins stored in granules will also respond faster.
The growth hormones all tend to bind to receptor tyrosine kinase that respond slower than many of the G-protein coupled receptors but the stimulated effect also takes longer to shut down than the GPCR effects.
Good luck
- Badges
- Science method
- Similar topics
- Biological Science
- Genetics
- Gene Expression
- Protein Expression