What are possible factors influencing the isotope-based hydrograph separation in river confluences?

When performing hydrograph separation (EMMA method) with isotope tracers in river confluences (natural large basin), we found that the contribution of each end member sometimes exceeds the...

20 January 2021 8,403 5 View

How can I simulate noise of CMOS Image Sensor by sentaurus TCAD?

Can anyone tell me how to simulate noise of CMOS image sensors, such as thermal noise and flicker noise with sentaurus TCAD?

09 November 2020 9,616 4 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Can I put one mouse in one cage?

I have three mice but I only need to scarify two in my experiment. Can I keep the leftover one mouse in the cage with itself? The other option is to put it in another cage with other mice. But...

12 July 2020 307 5 View

Why ammonium chloride an inhibitor for lysosome?

Ammonium chloride is a lysosome inhibitor and a weak base as described in a published paper, Ammonium chloride, an inhibitor of phagosome-lysosome fusion... . However NH4+ is a weak acid, I...

21 April 2020 8,419 3 View

How to design steel rack accorfing to ANSI MH 16.1: 2012?

Does some have got the commentary of the American norm ANSI MH 16.1: 2012? according to this norm the seismic design of the steel rack could be performed using R=4 as behavior factor, this value...

19 November 2019 6,598 2 View

Why I have very strong phospho protein signal in western blot?

I am working with phospho protein in western blot and have problems about the phospho protein signal. It is common that researchers can not detect phospho-protein signal in the membrane, as...

17 July 2019 6,027 3 View

How to decontaminate a 2D materials on TEM grid for atomic scale imaging?

I was trying to imaging the 2D materials with aberration corrected TEM, including MoS2, ReS2 and graphene. However, I found serious carbon contamination when zoom in at the region of interest....

30 May 2019 9,828 3 View

How to use software Geneious to find genomic features?

How to use software Geneious to find genomic features such as genome size, GC content, tRNA genes, CDS etc?

10 June 2017 1,062 1 View

How canI verify the DNA fragment without results after blast in NCBI?

15 January 2017 4,595 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View