How to get rid of significance issues in Western blot quantification of irregularly expressed proteins between samples?

Some proteins (abnormal proteins!?) expression pattern is highly different among individual sample so that it is very hard to get the significance difference between the group. For example, the...

27 January 2021 3,629 4 View

I am finding a lot of cellular debris in the culture flask? What are the reason behind it?

I am facing a problem that cellular debris observed in my SHSY5Y cells. I have seeded the cells in DMEM media with 10% FBS in T-25/ 60mm dish (antibiotic used PEN-Strepand gentamycin). I have also...

17 January 2021 3,074 3 View

How can we calculate theoretical stacking fault energy of unknown/new complex alloys?

Is there any method/theoretical approach to calculate the stacking fault energy of complex alloys such as high entropy alloys? The theoretical/ approximate value of SFE is needed for alloy design.

16 November 2020 7,074 3 View

How much sample size is necessary for the cross sectional study done in school/college level students about their smoking and drinking behaviors?

26 September 2020 8,101 7 View

How to fill the gap between two contigs?

Hi everyone, We have sequenced genome of data of a comparatively less studied species using Pacbio long read sequencer. Now after initial assembly we compared the contigs with previous version...

25 June 2020 3,442 3 View

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

How ultrasound and microwave can be effective factors on cotton and wool fabric which help the fabric to improve its dye uptake ability?

15 June 2020 4,541 3 View

How COVID19 would affect the other research areas in Biology?

As the whole world is suffering with COVID19 infection; the research for vaccines are at its peak and so is the funding towards virology projects. Will it have a negative impact on funding in...

25 May 2020 8,579 3 View

How to make ROC plot for the validation of ligand-based-pharmacophore models in discovery studio?

Hello, I already made a ligand-based pharmacophore by utilizing 60 dataset compounds by catalyst module in discovery studio. But I want to explore the ROC curve of that pharmacophore in order to...

02 March 2020 9,044 5 View

How to solve the GROMACS error: The initial cell size (0.657461) is smaller than the cell size limit (0.683200)?

I am simulating a glycerol-water mixture system in a square box of length 3.5 nm. After the NPT step my box size decreased to 2.62984 nm. However, in the beginning of the production run step I...

27 February 2020 2,830 1 View

Can I import Abaqus simulated file to Ansys Workbench for further simulation?

I am using Abaqus/CAE for Laser Shock Processing (LSP), Can I import the simulated model file to Ansys Workbench for further analysis as I have to run two simulation for fatigue, FOD damage...

19 October 2019 7,031 2 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

How the standard hydrogen electrode value is convertible into reversible hydrogen electrode?

As much I remember +0.0592*pH, as I am working on CatMAP, so my voltage values are in SHE, not RHE.

18 February 2021 1,685 3 View

Can explain this diagram to me, is this the average consumption curve for one day or the total consumption of days in a month summed up in specific?

The hourly load curves of the selected household in each season are shown in Fig. 28. Figure 28 shows the demand for electricity in four months on an hourly basis. Can you explain this diagram to...

08 February 2021 657 16 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Can anyone suggest a numerical method to calculate perimeter of a contour in charge density contour maps?

Hi i have drawn the charge density contour maps using VESTA taking CHGCAR files from VASP. Now, What I Want is that I need to know the perimeter of each contour appearing in the cross section 2D...

04 February 2021 3,637 1 View

Which one is better for storing energy: Fuels synthesized from CO2 or Hydrogen?

Based on educated guesses, which one of the following promises a better future for storing energy for grid scale, automobiles etc as opposed to batteries: 1) Fuels synthesized form CO2 2)...

02 February 2021 8,741 6 View

Why is WST-1 assay giving inconsistent result?

i want to determine the cytotoxicity activities of some drugs with WST-1 assay using 293T cell lines in 96 well plate. I usually seed 10,000 cell per well and incubate for ~48 hours. I expected...

01 February 2021 8,554 3 View