How long do you think we can keep stem cells frozen in liquid nitrogen and still be able to use it in patients for stem cell transplant? I can't many literature on this, some I have come across suggests forever? Surely eventually it will be clinically unfit for purpose?
hello it should be as long as it is preserved in liquid nitrogen
Papers by Hal Broxmeyer on umbilical cord blood cells offer some answers.
Article High-efficiency recovery of functional hematopoietic progeni...
Article High-efficiency recovery of immature haematopoietic progenit...
Article Hematopoietic stem/progenitor cells, generation of induced p...
Article High efficiency recovery of hematopoietic progenitor cells w...
The PBMCs stored in PVC bags exhibited no loss of CFU-GEMM activity after 1 to 1.5 years, but a 40-percent loss of activity was observed after 2 to 2.4 years. PBMCs stored in polyolefin bags, however, exhibited no loss of CFU-GEMM activity, even after 2 to 2.4 years of storage. In vitro recovery was significantly lower in PBMCs stored in vials at -80 degrees C or -135 degrees C than in cells stored in PVC or polyolefin bags at these temperatures, both in the 1- to 1.5-year and the 2- to 2.4-year time frames. In vitro recovery and viability were similar in PBMCs stored in vials at -80 degrees C, -135 degrees C, -150 degrees C, and -197 degrees C. The growth patterns in the CFU-GEMM assay in PBMCs stored in vials were significantly lower after storage at -80 degrees C than after storage at -135 degrees C, -150 degrees C, or -197 degrees C.
Dear Nazia
You can keep stem cells frozen for and still use them for transplant in patients as follows or any other viable cell
1. At – 20 C up to 3 months
2. At -40 C up to 1 year ( Frozen temperature )
3. At -80 C up to 3 years (Cryo temperature)
4. At -173 C up to 9 years (Liquid Nitrogen Temperature)
5. At – 273 C life long (Absolute Zero Temperature)
Hopefully It may help you
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