I am doing DNA extraction with the Phenol-Chloroform protocol. In the end, I will need to resuspend this DNA in ultrapure water for DNA concentration. As a matter of time, I would like to know if there is any problem in storing the DNA in isopropanol (precipitation step) for a long time (days) at -20 °. What would be the best way to store it at -20 °, for about 4 days, in this situation?
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Yuri F. Drygin
Dear Elisa,
next step after adding isopropanol is centrifugation early or late. Why not to collect DNA by centrifugation, wash it with 70% ethanol and keep at -20*C for years?
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Michael J. Benedik
Agree with the above responses. There is no issue in storing at -20 in isopropanol other than the increased co-precipitation of contaminants.
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