Hi,
I am studying some carbapenem resistant clinical isolates of Klebsiella. The resistance is probably due to plasmids that carry the resistance genes. These genes are usually found in insertion sequences and transposable elements. Now plasmids carrying these resistance genes are reported to be self-conjugative in literature. I performed conjugation experiments and analysed next generation sequencing results coming from the strains having these plasmids. Anyway the conjugation did not work and I can't find the genes involved in the conjugation (I searched for Tra and Mob genes) on the sequences of my contigs. Is it possible that my plasmids are not conjugative and they acquired the resistance genes by transposition of a gene which was located on a conjugative plasmid?I am studying carbapenem resistant clinical isolates of an outbreak . So, I was wondering how the resistance could have been spread if these plasmids are not transferrable.
Thank you
Hi Silvia,
I think this article may highlights your question:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652767/
Hi Silvia,
I think this article may highlights your question:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652767/
I think the transposons are responsible in this cases and may by transfer by chromosomal DNA through cell reproduction
My main guesses for this would be:
I strongly agree with Alex. I suggest you look at other horizontal gene transfer mechanisms especially transduction. A very strong opinion is the transposon-directed chromosomal transfer. Good luck
Plasmids are extrachromosomal genetic elements capable of autonomous replication. I think you should also factor the phenotypic effects and resistance transfer factor (classification of plasmids) as far as classification of plasmids is concerned and their roles. Find also if episomes plays a role here.
Transposable genetic elements are segments of DNA that have the capacity to move from one location to another (i.e. jumping genes), due to genetic instability of phenotypes (IS elements and Tn elements).
I concur Jai Gosh.
yes may be transferred by another technique such as transduction
If you haven't done it, I suggest you read our review Nat Rev Microbiol. 2005 Sep;3(9):722-32, a bit old now but most HGT mechanisms have been described many years ago.
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