Alexandra Johnson

3 Questions 146 Answers 0 Followers

Questions related from Alexandra Johnson

Keeping cover slips from sliding around when growing cells on top of them?

I'm growing RAW 264.7 cells on poly-l-lysine coated cover slips in 6 well plates, infecting them with bacteria, then doing May Grunwald Giemsa staining on them. Following this, they're being...

10 October 2017 1,357 4 View

Have any protocols or tips for biparental mating of E. coli with gram positive organisms as the recipient?

I'm going to attempt a biparental mating of E. coli S17-1λpir (donor) with a few Enterococcus faecalis isolates (recipient). I've come across some publications that mention the RK2/RP4 transfer...

04 April 2017 2,806 1 View

Does anyone have E. coli strain MDS42 ΔrecA trfA Blue?

I tried ordering it from Scarab Genomics but they said they discontinued it.

01 January 2017 9,202 3 View